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KMID : 0364820070430030232
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Korean Journal of Microbiology
2007 Volume.43 No. 3 p.232 ~ p.235
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Identification of the Nitrifying Archaeal Phylotype Carrying Specific amoA Gene by Applying Digital PCR
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Park Byoung-Jun
Park Soo-Je
Rhee Sung-Keun
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Abstract
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Mesophilic Crenarchaeota have been known to be predominant among ammonia-oxidizing microorganisms in terrestrial and marine environments. In this study, we determined the archaeal phylotypes carrying specific amoA by combining digital PCR and multiplex-nested PCR. Analysis of samples in which amoA and 16S rRNA gene were amplified showed that amoA gene diversity was relatively higher than that of 16S rRNA gene. Nitrifying archaeal group I.1a was dominant over I.1b group of crenarchaota and euryarchaeota. This approach could be applied for interrelating a functional gene to a specific phylotype in natural environments.
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KEYWORD
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amoA, digital PCR, diversity, nitrifying archaea, tidal flat, 16S rRNA gene
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